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Electrophoresis PDMS/glass chips with continuous on-chip derivatization and analysis of amino acids using naphthalene-2,3-dicarboxaldehyde as fluorogenic agent |
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| Authors: | Yassine O Morin P Dispagne O Renaud L Denoroy L Kleimann P Faure K Rocca J-L Ouaini N Ferrigno R |
| Institution: | a Institut des Nanotechnologies de Lyon, INL, CNRS UMR5270, Université de Lyon, F-69003 Lyon, France b Université Lyon 1, Villeurbanne F-69622, France c Laboratoire de Neuropharmacologie et Neurochimie, FRE CNRS 3006, Institut Fédératif des Neurosciences de Lyon IFR 19, Université de Lyon, Lyon F-69003, France d Université Lyon 1, Lyon F-69373, France e Laboratoire des Sciences Analytiques, LSA, CNRS UMR 5180 Université de Lyon, Lyon F-69003, France f Faculté des Sciences et de Génie Informatique, Université Saint Esprit de Kaslik, BP 446 Jounieh, Lebanon |
| Abstract: | In this work, we developed a PDMS electrophoresis device able to carry out on-chip derivatization and quantification of amino acids (AAs) using naphthalene-2,3-dicarboxaldehyde (NDA) as a fluorogenic agent. A chemical modification of the PDMS surface was found compulsory to achieve the derivatization of AAs with NDA and a limit of detection (LOD) of 40 nM was reached for glycine. Finally, we suggested the applicability of this microdevice for the analysis of real biological samples such as a rat hippocampus microdialysate. |
| Keywords: | Capillary electrophoresis microchip Microfluidic Laser-induced fluorescence detection Derivatization Amino acids |
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