| 连续棒状弱阳离子交换柱的合成及其对蛋白质保留行为 |
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| 引用本文： | 卫引茂,黄晓冬,陈强,耿信笃.连续棒状弱阳离子交换柱的合成及其对蛋白质保留行为[J].分析化学,2000,28(10):1194-1198. |
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| 作者姓名： | 卫引茂 黄晓冬 陈强 耿信笃 |
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| 作者单位： | 1. 西北大学现代分离科学研究所,现代分离科学省重点实验室,西安,710069
2. 宝鸡文理学院 |
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| 基金项目： | 国家自然科学基金!（批准号：29675017和39880003）,陕西省教委科学基金资助课题 |
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| 摘 要： | 以甲基丙烯酸缩水甘油酯为单体,乙二醇二甲基丙烯酸酯为交联剂在空管柱内就地聚合制备了一种聚合物连续棒状色谱基质,并通过“在线”化学改性将其修饰为弱阳离子交换柱。考察了该色谱柱的孔结构特征、表面亲水性能、对标准蛋白的分离效果和ｐＨ值对保留行为的影响以及色谱柱的重现性。结果表明,该色谱柱对蛋白的分离性能及重现性良好,柱寿命不短于半年。对溶菌酶的活性回收率达９６．２％。流动相流速为８．０ｍＬ／ｍｉｎ条件下
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| 关 键 词： | 连续棒状色谱柱 弱阳离子交换 蛋白质 分离 |
Preparation of Continuous Rod Column of Weak Cation Exchange and Its Chromatographic Behaviour for Proteins |
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| Wei Yinmao,Huang Xiaodong,Chen Qiang,Geng Xindu.Preparation of Continuous Rod Column of Weak Cation Exchange and Its Chromatographic Behaviour for Proteins[J].Chinese Journal of Analytical Chemistry,2000,28(10):1194-1198. |
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| Authors: | Wei Yinmao Huang Xiaodong Chen Qiang Geng Xindu |
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| Institution: | Wei Yinmao
,Huang Xiaodong
,Chen Qiang
,Geng Xindu
;(Institute of Modern Separation Science, Key Laboratory of Modern Separation Science in Shaanxi Province, Northwest University, Xi'an 710069) |
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| Abstract: | A continuous rod column of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) was prepared by direct polymerization inside an empty chromatographic column.It was chemically modified for a weak cation exchanger by a "in-situ" method. This rod column was evaluated for its pore property, hydrophilicity, separation ability to protein,effect of pH on retention and reproducibility. It was found that die column exhibited a high activity recovery of 96. 2%, low column back pressure and excellent reproducibility. Five proteins weirs well separated within 3.5min using the column. The life time of the synthesized column was tested to ha at least longer than sit months. |
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| Keywords: | Continuous rod column weak cation exchange biopolymer rapid separation |
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