基于含氟表面活性剂修饰的金纳米粒子测定卡托普利

在较高离子强度和一定温度下,卡托普利能引起非离子表面活性剂FSN-100修饰的14nm金纳米粒子胶体溶液快速聚集,引起金纳米粒子在519nm处的吸光度降低,而在640nm处的相对吸光度成线性增加.据此,建立了一种光度测定卡托普利的新方法.卡托普利药片制剂中常见赋形剂,如淀粉、糊精、葡萄糖、果糖、麦芽糖、蔗糖、明胶、山梨醇、乳糖等对本实验没有干扰.本方法具有快速、简便、选择性高等优点,线性范围为2.0～12.5μg/mL,卡托普利检出限(S/N=3)为1.25μg/mL.该方法成功用于药片制剂中卡托普利的测定,回收率在99%到103%之间.

Detection of captopril via fluorosurfactant-capped gold nanoparticles

In the presence of high ionic strength（50 mM NaCl） and high temperature（40 ℃）,captopril may cause the aggregation of the nonionic fluorosurfactant（FSN-100）-capped 14 nm gold nanoparticles（GNPs） and led to the red-shift of the surface plasmon resonance absorption peak.Based on this phenomenon,a rapid spectrophotometry method for captopril has been developed.The typical interference species in pharmaceutical formulations,such as starch,dextrin,glucose,fructose,maltose,sucrose,gelatine,sorbitol,and lactose did not result in the aggregation of gold colloidal solution.The present approach possessed various advantages,such as simple,rapid and high selective.The calibration curve is linear over the range of 2.0？12.5 μg/mL for captopril,the detection limit（S/N=3） is 1.25 μg/mL.This method has been successfully used for detection of captopril in tablets,and the recoveries of captopril were in the range between 99% and 103%.