甲醇和乙醇的三维荧光光谱特性研究

为阐明甲醇和乙醇的光谱学特性,建立两者快速的辨析方法。采用三维荧光光谱,对甲醇和乙醇的荧光特性进行了研究。分析结果显示,甲醇的三维荧光光谱中出现了两个特征荧光峰,其荧光强度在甲醇体积浓度小于15%时与其浓度成正相关,乙醇的三维荧光光谱中出现了一个完整的特征荧光峰,其荧光强度在乙醇体积分数小于50%时与乙醇浓度成相关,甲醇比乙醇具有更高的荧光效率,以甲醇为有机溶剂研究化合物的荧光特性时,需要考虑甲醇的本底荧光。甲醇和乙醇的特征荧光峰位置差别较大,前者特征荧光峰出现在225/35 0nm和250/375nm处,而后者特征荧光峰位于240/310nm,据此可以有效辨析两种醇。

Study on the characteristics of three-dimensional fluorescence excitation-emission spectra of methanol and ethanol

For the purpose of revealing the spectra characteristics of methyl and ethanol, and establishing a method for distinguishing each other, the fluorescence features of methyl and ethanol were studied by three-dimensional fluorescence excitation-emission matrix spectra. The results obtained showed that there were two peaks in the three-dimensional fluorescence excitation-emission matrix spectra of methyl, and the intensities of the two peaks were positively related to the concentration of methyl when it was less than 15%. On the other hand, a whole fluorescence peak was only observed in the three-dimensional fluorescence excitation-emission matrix spectra of ethanol, and the intensity of the peak was positively correlated to the content of ethanol when it was less than 50%. There was a higher fluorescence efficiency for the methanol as compared to the ethanol. When the methyl was used for organic solvents to study the fluorescent nature of the organic matter, the fluorescence emitted by the methyl should be deduced. The locations of the fluorescence peaks of the methyl and ethanol were different. The peaks of the methyl were located at 225/350 nm and 250/375 nm, while the peak of the ethanol was characterized by 240/310 nm. Therefore, the fluorescence peak locations of the two alcohols could be applied to discriminate each other.