超顺磁性DNA纳米富集器应用于痕量寡聚核苷酸的富集

随着纳米技术的迅速发展 ,纳米材料逐渐被应用到细胞生物学和分子生物学研究领域 ,为生物医学的研究和发展提供了新的技术和手段 [1～ 4 ] .如超顺磁性纳米颗粒由于具有尺寸小、比表面积大、悬浮稳定性好及在外磁场作用下的磁导向性运输和富集等优良特性 ,使其在细胞和生物活性

Concentration of Trace Amounts Oligonucleotide Using Super-paramagnetic DNA Nano-enricher

The present paper covers a novel technology for the concentration of trace amounts of target oligonucleotide from the solution. This technique is based on a super-paramagnetic DNA nano-enricher constructed with a single strand DNA probe immobilized onto the surface of the super-paramagnetic nanoparticles prepared by using the water-in-oil microemulsion technique, employing silica as the shell and iron oxide as the core of the super-paramagnetic nanoparticles. The silica coated magnetic nanoparticles are (40±4) nm in size. And the magnetic nanoparticle is super-paramagnetic. Biotin labeled ssDNA(Biotin-5-(A)10-GAT-TCA-CGA-GGC-CCT-AGT-CG-3') was immobilized on the surface of silica coated magnetic nanoparticles. The complementary ssDNA could be enriched effectively and the characteristics of the enriched ssDNA have not changed, which will provide a novel technique and measurement for gene transfection, mutation detection, gene diagnosis, gene therapy and so on.