Detection of residual bacitracin A, colistin A, and colistin B in milk and animal tissues by liquid chromatography tandem mass spectrometry |
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Authors: | Eric Chun-hong Wan Clare Ho Della Wai-mei Sin Yiu-chung Wong |
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Institution: | (1) Analytical and Advisory Services, Government Laboratory of the Hong Kong Special Administrative Region (HKSAR), Homantin Government Offices, Hong Kong, China |
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Abstract: | Liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-MS/MS) was applied to the determination
of residual bacitracin A, colistin A, and colistin B in milk and animal tissue samples. Prior to instrumental analysis, samples
were subjected to acid extraction followed by solid-phase cleanup using Strata-X cartridges. Mass spectral acquisitions were
performed under selective multiple reaction monitoring (MRM) mode at m/z 199 and 670 from triply charged precursors of bacitracin A (m/z 475); m/z 385 and 379 from triply charged precursors of colistin A (m/z 391); and m/z 380 and 374 from triply charged precursors of colistin B (m/z 386). Method precision was evaluated from spike recovery of samples fortified at concentrations corresponding to 2/5 of the
maximum residue limits (MRLs) for each of the analytes under study. Intra-day and inter-day variations were found to range
from 90.9 to 104% with relative standard deviation (RSD) <6.5%, and from 90.1 to 106% with RSD <9.1%, respectively. Limits
of quantification (LOQs) were defined as the spiking concentrations at 2/5 MRL, and limits of detection (LODs) were 10–47 μg kg−1 for bacitracin A, 1–16 μg kg−1 for colistin A, and 6–14 μg kg−1 for colistin B in milk and animal tissues. The presented method has good precision and high sensitivity and was applied as
a fast screening protocol and a quantitative tool for monitoring of the concerned polypeptides in foods as part of a surveillance
program.
![MediaObjects/216_2006_325_Figa_HTML.jpg](/content/2j6755n73788302h/MediaObjects/216_2006_325_Figa_HTML.jpg) |
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Keywords: | Bacitracin colistin milk Animal tissues LC-MS/MS |
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