Examination of dye-protein interaction by gel-permeation chromatography |
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| Authors: | Sereikaite Jolanta Bumelis Vladas-Algirdas |
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| Institution: | Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, Sauletekio al. 11, LT-2040, Vilnius, Lithuania. sjolanta@fm.vtu.it |
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| Abstract: | The interaction of Cibacron blue F3G-A with two therapeutic proteins, recombinant human growth hormone and recombinant human interferon-alpha2b, has been examined by applying gel-permeation chromatography in combination with the absorption difference spectroscopy. The complexes of these proteins with Cibacron blue F3G-A have been isolated, and their absorbance spectra have been registered. The influence of Cibacron blue F3G-A on the oligomeric state of proteins has been investigated. It was found that Cibacron blue F3G-A promotes the generation of interferon-alpha2b dimers at pH 5.0. |
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| Keywords: | Cibacron blue F3G‐A recombinant human growth hormone recombinant interferon‐α2b dye–protein interaction |
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