Estimation of the minimum uncertainty of DNA concentration in a genetically modified maize sample candidate certified reference material |
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Authors: | J Prokisch R Zeleny S Trapmann L Le Guern H Schimmel G N Kramer J Pauwels |
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Institution: | (1) EC-JRC-IRMM, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Retieseweg, Geel 2440, Belgium e-mail: jozsef.prokisch@irmm.jrc.be, BE |
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Abstract: | Homogeneity testing and the determination of minimum sample mass are an important part of the certification of reference
materials. The smallest theoretically achievable uncertainty of certified concentration values is limited by the concentration
distribution of analyte in the different particle size fractions of powdered biological samples. This might be of special
importance if the reference material is prepared by dry mixing, a dilution technique which is used for the production of the
new and third generation of genetically modified (GMO) plant certified reference materials. For the production of dry mixed
PMON 810 maize reference material a computer program was developed to calculate the theoretically smallest uncertainty for
a selected sample intake. This model was used to compare three differently milled maize samples, and the effect of dilution
on the uncertainty of the DNA content of GMO maize was estimated as well. In the case of a 50-mg sample mass the lowest achievable
standard deviation was 2% for the sample containing 0.1% GMO and the minimum deviation was less than 0.5% for the sample containing
5% GMO.
Received: 5 December 2000 / Revised: 14 March 2001 / Accepted: 19 March 2001 |
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