| 三种不同光谱学方法测定转基因抗虫棉组织营养元素含量 |
| |
| 引用本文： | 孙彩霞,张玉兰,孙玉全,杨磊,王杰,崔振波. 三种不同光谱学方法测定转基因抗虫棉组织营养元素含量[J]. 光谱学与光谱分析, 2009, 29(11): 3038-3041. DOI: 10.3964/j.issn.1000-0593(2009)11-3038-04 |
| |
| 作者姓名： | 孙彩霞 张玉兰 孙玉全 杨磊 王杰 崔振波 |
| |
| 作者单位： | 1. 东北大学理学院,辽宁,沈阳,110004
2. 中国科学院沈阳应用生态研究所,辽宁,沈阳,110016 |
| |
| 基金项目： | 国家自然科学基金项目，辽宁省博士启动基金项目，辽宁省科技攻关项目 |
| |
| 摘 要： | 采用火焰原子吸收、火焰原子发射光谱法及分光光度法分析了三种不同转基因抗虫棉在不同生育时期不同绢织内的11种营养元素含量的变化.结果表明,与非转基因对照比较,转基因抗虫棉植株体的营养化学组成发生了一定程度的改变,这种改变与转基因抗虫棉的品种、部位及生育时期相关.转基因抗虫棉全N含量的变化最为显著,其中转Bt抗虫棉Z30增幅最高达21%,这可能与外源基因导入及杀虫蛋白的表达有关.Mg,Na和Cu含量的显著改变仪特异的发生在某个转基因抗虫棉品种的某一组织内.外源抗虫基因导入对转基因抗虫棉植株内有机C、全P、全S、K、Ca、Fe、Zn含量的扰动是微小的,从而推测,转基因抗虫棉对这些营养元素的利用和积累能力没有显著改变.
|
| 关 键 词： | 转基因抗虫棉 光谱学方法 营养元素 |
| 收稿时间： | 2008-11-06 |
Determination of Nutrient Elements in Transgenic Insect-Resistant Cotton Tissues by Three Different Spectroscopical Methods |
| |
| SUN Cai-xia,ZHANG Yu-lan,SUN Yu-quan,YANG Lei,WANG Jie,CUI Zhen-bo. Science College,Northeastern University,Shenyang ,China. Determination of Nutrient Elements in Transgenic Insect-Resistant Cotton Tissues by Three Different Spectroscopical Methods[J]. Spectroscopy and Spectral Analysis, 2009, 29(11): 3038-3041. DOI: 10.3964/j.issn.1000-0593(2009)11-3038-04 |
| |
| Authors: | SUN Cai-xia ZHANG Yu-lan SUN Yu-quan YANG Lei WANG Jie CUI Zhen-bo. Science College Northeastern University Shenyang China |
| |
| Affiliation: | SUN Cai-xia1,ZHANG Yu-lan2,SUN Yu-quan1,YANG Lei1,WANG Jie1,CUI Zhen-bo11. Science College,Northeastern University,Shenyang 110004,China2. Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China |
| |
| Abstract: | In order to find out the effects of exogenous genes,such as Bt and Bt coupled with CpTI,on nutrition metabolism in transgenic plants,totally eleven types of nutrient elements in transgenic Bt (Z30) and Bt-CpTI (CCRI41 and SGK321) cotton were determined using methods of flame atomic absorption spectroscopy,flame atomic emission spectroscopy and spectrophotometry at flowering stage and boll-opening stage. The results showed that the chemical composition of plant nutrition in transgenic insect-resistant cotton... |
| |
| Keywords: | Transgenic insect-resistant cotton Spectroscopy methods Nutrient elements |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
