牛磺酸对缺血/再灌注大鼠肾脏GRP78和Caspase-12表达的影响

观察牛磺酸(Tau)对肾缺血/再灌注(I/R)大鼠肾脏GRP78、Caspase-12表达的影响及其意义。将Wistar大鼠30只随机分为假手术组(对照组)、I/R组和I/R+TMP组。采用夹闭双侧肾蒂45min再灌注24h制备肾I/R模型。I/R+TMP组在手术前1h按200mg.kg-1腹腔注射Tau,余操作同I/R组。检测血清尿素氮(BUN)和肌酐(Cr);光镜观察肾小管组织结构变化;RT-PCR和免疫组化检测GRP78、Caspase-12mRNA和蛋白表达。与假手术组比较,I/R组大鼠血清BUN,Cr水平显著升高,肾组织损伤严重,GRP78和Caspase-12mRNA和蛋白表达呈显著性增加(P<0.01);与I/R组比较,I/R+Tau组血清BUN、Cr水平及GRP78、Caspase-12表达均有显著性下降(P<0.05),肾脏损伤明显减轻。牛磺酸对肾脏缺血/再灌注大鼠GRP78、Caspase-12过度表达升高有很好的抑制作用,这可能是它减轻肾脏缺血/再灌注损伤的重要机制之一。

Effect of taurine on expression of gene GRP78 and Caspase-12 of renal tissue at rats in ischemia reperfusion injury

The effects of taurine(Tau) on expressions of GRP78,Caspase-12 of the renal tissue at rats in renal ischemia /reperfusion(I/R) injury was observed.Thirty Wistar rats of either sex were randomly divided into sham operation group(control),I/R group and I/R+Tau group.The animal model of acute renal I/R injury was prepared by clamping bilateral renal pedicle for 45 mins and then reperfusion over 24 hours.I/R+Tau group was treated with intraperitoneal injection of 200 mg·kg-1 Tau solution 1 hour before surgery,and the rest operations followed the same as that in I/R group.The blood urea nitrogen(BUN) and creatinine(Cr) were detected.The histological changes of renal were observed by HE staining;the mRNA and protein expressions of GRP78.Caspase-12 in the renal tissue were analyzed by RT-PCR and immunohistochemistry,respectively.Compared with the Sham group,the serum BUN,Cr in I/R group had significant increases,the damage of renal tissue was much severer,the mRNA and protein expressions of GRP78 and Caspase-12 were significantly increased(p<0.01) as well.Contrast to the I/R group,the serum BUN,Cr level and the mRNA and protein expressions of GRP78 and Caspase-12 in I/R+Tau group dropped dramatically(p<0.05),the damage of renal tissue was lower.Hence,taurine could attenuate renal I/R injury,and might be one of the protective mechanism by down-regulating the over expressions of genes GRP78 and caspase-12.