| 反义基因逆转肿瘤细胞多药耐药性和诱导细胞凋亡的研究 |
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| 引用本文： | 张凤春,林玉梅,姜玉珍,卢振霞,孙延霞,张秀梅.反义基因逆转肿瘤细胞多药耐药性和诱导细胞凋亡的研究[J].暨南大学学报,1998(6). |
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| 作者姓名： | 张凤春 林玉梅 姜玉珍 卢振霞 孙延霞 张秀梅 |
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| 作者单位： | 白求恩医科大学第三临床学院血液、肿瘤科 |
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| 摘 要： | 探讨克服肿瘤细胞多药耐药（ＭＤＲ１）性的方法，提高化疗效果。本文采用多药耐药反义基因（ＭＤＲ１－ＲＳＰＳ－ＯＤＮ）逆转Ｋ５６２／ＡＤＭ肿瘤细胞的ＭＤＲ１，诱导肿瘤细胞凋亡，发现ＭＤＲ１－ＡＳＰＳ－ＯＤＮ诱导Ｋ５６２／ＡＤＭ细胞株细胞产生大量ＤＮＡ断片，ＦＡＣＳ检测发现几乎全部ＭＲＤ＋Ｋ５６２／ＡＤＭ细胞发生凋亡。其结果表明ＤＭＤＲ１－ＡＳＰＳ－ＯＤＮ能有效、特异地抑制ＭＤＲ１基因表达，逆转肿瘤细胞的ＭＤＲ１，促进阿霉素诱导ＭＤＲ＋１Ｋ５６２／ＡＤＭ细胞凋亡，为其临床应用提供理论依据
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| 关 键 词： | 反义基因 多药耐药 细胞凋亡 |
Study of Antisense Oligodeoxynucleotide in Reversing Multidrug Resistance and Inducing Apoptosis of Tumor Cells |
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| Zhang Fengchun,Lin Yumei Jiang Yuzhen Lu Zhenxia Sun Yanxia\ Zhang Xiumei.Study of Antisense Oligodeoxynucleotide in Reversing Multidrug Resistance and Inducing Apoptosis of Tumor Cells[J].Journal of Jinan University(Natural Science & Medicine Edition),1998(6). |
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| Authors: | Zhang Fengchun Lin Yumei Jiang Yuzhen Lu Zhenxia Sun Yanxia\ Zhang Xiumei |
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| Abstract: | Objective:To explore an approach to the reversal of multidrug resistance(MDR)of tumor cells.Methods: Antisense Oligodeoxynucleotide (mdr-L-AS PS-ODN)was used.Results:A Large amount of DNA fragments was found in mdr-1-AS PS-ODN treated K562/ADM cells and almost all the mdr-1 K562/ADM cells underwent apoptosis.Conclusion:mdr-1-AS PS-ODN could inhibit specifically mar-1 gence expression, effectively reserse MDR of the tumor cells and promote ADM induced apoptosis of mdr-1 K562/ADM |
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| Keywords: | antisense oligodeoxynucleotide multidrug resistance apoptosis |
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