| 小菜蛾颗粒体病毒DNA限制性酶切分析及基因组DNA片段克隆 |
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| 引用本文： | 孟小林,肖红,吕颂雅,徐进平,叶林柏,ＳｕｚａｎｎｅＭ．Ｔｈｉｅｍ. 小菜蛾颗粒体病毒DNA限制性酶切分析及基因组DNA片段克隆[J]. 武汉大学学报(理学版), 1997, 0(6) |
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| 作者姓名： | 孟小林 肖红 吕颂雅 徐进平 叶林柏 ＳｕｚａｎｎｅＭ．Ｔｈｉｅｍ |
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| 作者单位： | 武汉大学病毒研究所 |
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| 摘 要： | 小菜蛾颗粒体病毒ＤＮＡ用ＢａｍＨＩ和ＸｈｏＩ酶切，经０．７５％琼脂糖凝胶电泳，分别得到１２条带和８条带，平均分子量为１１３．０ｋｂ．用Ｓｕｐｅｒｃｏｓ１Ｃｏｓｍｉｄ作载体，将Ｓａｕ３ＡＩ部分消化的ＰｘＧＶ－ＤＮＡ随机片段插入该载体的ＢａｍＨＩ酶切位点，转化于ＸＬ１－Ｂｌｕｅ受体菌，经Ａｍｐ平板筛选转化子，挑出２５６个Ａｍｐ＋菌落，以３２Ｐ－ｄＣＴＰ标记的ＰｘＧＶ－ＤＮＡ为探针，斑点杂交筛选得到５９个阳性重组体，再经电泳检测，得到了５９个不同大小的ＰｘＧＶ－ＤＮＡ片段克隆．
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| 关 键 词： | 小菜蛾颗粒体病毒，酶切图谱，DNA片段克隆 |
THE ANALYSIS OF RESTRICTION ENZYME OF PLUTELLA XYLOSTELLA GRANULOSIS VIRUS DNA AND CLONING OF DNA FRAGMENTS |
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| Meng Xiaolin,Xiao Hong,Lu Songya,Xu Jinpin,Ye Linbai. THE ANALYSIS OF RESTRICTION ENZYME OF PLUTELLA XYLOSTELLA GRANULOSIS VIRUS DNA AND CLONING OF DNA FRAGMENTS[J]. JOurnal of Wuhan University:Natural Science Edition, 1997, 0(6) |
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| Authors: | Meng Xiaolin Xiao Hong Lu Songya Xu Jinpin Ye Linbai |
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| Abstract: | Plutella xylostella Granulosis Vinus(PxGV)DNA was cut by BamHI and Xhol and electrophoresed in 0.75% agarose gel, producing 12 and 8 fragments respectively. The average molecular weight of PxGV DNA 113.0 kb. PxGV DNA was digested partially by Sau3Al, fragments were recombinants with Supercos 1 cosmid vector and transformated in the XL1 Blue.256 transformants were selected from ampicilin plate,59 positive recombinans were selected by dot blot using genome PxGV DNA 32 P dCTP labelled as probe and constructed 59 different size PxGV DNA fragments colone by the examination of agarose gel. |
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| Keywords: | Plutella xylostella granulosis virus restrction map of PxGV DNA DNA fragment clone |
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