海洋深层水与褐藻糖胶配伍应用抑制HepG2肝细胞脂质积聚调节作用的研究

以Hep G2细胞为模型,研究海洋深层水(deep sea water,DSW)与褐藻糖胶(fucoidan,FPS)配伍应用对Hep G2细胞脂质代谢的调节作用。采用MTT法确定与DSW配伍应用的FPS浓度控制在50μg/m L以内。在这一范围内,随着FPS浓度的增加(分别为5,10,20,50μg/m L),Hep G2细胞胞内的脂质含量逐渐降低,呈现明显的剂量依赖关系。而且,DSW与50μg/m L的FPS配伍应用的效果显著优于单用同剂量的DSW或FPS。实验结果表明,DSW与FPS配伍应用对Hep G2细胞脂质代谢具有更好的调节作用,其最佳配伍应用浓度为50μg/m L,这为高脂血症的防治提供了一个新的思路。

Matched application of deep sea water and fucoidin in attenuation of lipid accumulation in HepG2 cells

We addressed regulation effect of matched application of deep sea water (DSW) and fucoidan (FPS) on lipid metabolism with HepG2 cells as models. We confirmed the concentration of FPS matched with DSW should be controlled within 50 μg/mL by MTT assay. Within this scope, lipid content of HepG2 cells gradually decreased with the increase of FPS concentration (5, 10, 20, and 50 μg/mL). Significant doze dependent phenomenon exists. Moreover, matched application of DSW and 50 μg/mL FPS was significantly superior to single 50 μg/mL DSW or FPS.Experimental results show that matched application of DSW and FPS has better regulation effect to lipid metabolism of HepG2 cells, optimal matched application concentration of 50 μg/mL. This research provides a new solution for prevention and treatment of hyperlipidemia.