紫膜菌紫质的拉曼光谱测试

菌紫质是一种可进行光信息存储与能量转换的生物膜蛋白分子。在光化学循环中 ,它包含的视黄醛产生快速的全反——— 1 3顺式异构化并去质子 ,然后又重新质子化并在反应循环终了转为全反式构型。了解菌紫质的分子构造及光化学反应机制是很有意义的事 ,但由于菌紫质是紫色膜蛋白 ,加之光反应时的荧光问题 ,用普通拉曼技术获得菌紫质的特征峰有一定困难。一般都用时间分辨付里叶变换红外光谱来进行它的测试工作。文本采用传统拉曼技术 ,分别用 5 3 2、785和 1 0 64nm波长激光作激发光源 ,测试了菌紫质的拉曼光谱 ,并得到了菌紫质的大部分特征拉曼谱带。

Raman Spectra of Bacteriorhodopsin

Bacteriorhodopsin is a biological membrane protein with retinal as a prosthetic group. In the photochemical cycle, retinal changes its configuration from all-trans to 13-cis and becomes deprotonated, then again protonated and all-trans at the end of the reaction cycle. It is interesting in understanding for molecule structure and photochemical mechanism of bacteriorhodopsin. Purple membrane and fluorescence of photoreactions are problems encountered with traditional Raman spectroscopy. In general the bacteriorhodopsin is measured by time-resolved FTIR spectroscopy. In this paper the bacteriorhodopsin Raman spectra were obtained by using traditional Raman technique with 532,785 and 1064 nm wavelength of a continious wave laser as the excited resource. The data clearly show that Raman spectroscopy is a useful tool for the study of chromophore-containing biological systems.