Self-assembled nanozyme complexes with enhanced cascade activity and high stability for colorimetric detection of glucose

Construction of multi-enzyme complexes not only can help expand the understanding of biological mechanisms, but also holds great promise in biosynthesis, biosensing and biomedicine. Herein, a hybrid multi-enzyme system, CeO₂/glucose oxidase (GOx) nanocomplex was developed via self-assembly and exhibited excellent catalytic activity toward cascade reactions. Compared to mixed GOx and CeO₂, the nanocomplexes displayed enhanced efficiency, mainly attributed to the minimal diffusion of intermediate in the nanocomplexes system. Moreover, the nanocomplexes exhibited outstanding long-term stability and excellent recyclability. Benefiting from these merits, a highly sensitive and selective biosensor for colorimetric detection of glucose was constructed based on CeO₂/GOx nanocomplexes. Such a self-assembled nanozyme complex offers a simple and efficient example to build spatially confined multi-enzyme systems to potentiate their applications in energy conversion, detoxification and bioanalysis