The “Fingerprint” of a freshwater microalga Scenedesmus sp. LX1: Visualizing the composition of its soluble algal products |
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| Authors: | Yinhu Wu Yin Yu Hongying Hu |
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| Affiliation: | 1. Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, China;2. State Key Laboratory of Environmental Criteria and Risk Assessment, Research Center of Water Pollution Control Technology, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;3. Shenzhen Environmental Science and New Energy Technology Engineering Laboratory, Tsinghua-Berkeley Shenzhen Institute, Shenzhen 518055, China;1. Hangzhou Normal University, Hangzhou 310036, China;2. Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda 20892, United States;1. Key Laboratory of Polyoxometalate Science of Ministry of Education, Faculty of Chemistry, Northeast Normal University, Changchun, Jilin 130024, PR China;2. Faculty of Chemistry, Tonghua Normal University, Tonghua, Jilin 134002, PR China;1. College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China;2. State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin, China Institute of Water Resources and Hydropower Research, Beijing 100038, China;1. Botanical Institute Christian-Albrechts University Kiel, Olshausenstraße 40, DE 24098 Kiel, Germany;2. Biogeochemical Modelling, GEOMAR, Helmholtz-Zentrum für Ozeanforschung Kiel, Düsternbrooker Weg 20, 24105 Kiel, Germany;1. Department of Chemical Engineering, Laboratory of Advanced Materials (MOE), Tsinghua University, Beijing 100084, China;2. School of Physics and Nuclear Energy Engineering, Beihang University, Beijing 100191, China;1. School of Life Science, Anhui University, Hefei 230039, China;2. National Laboratory of Physical Sciences at Microscale and School of Life Science, University of Science and Technology of China, Hefei 230027, China;3. High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei 230031, China |
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| Abstract: | In this study, a fingerprint analysis method along with the EEM fluorescence spectra was used to characterize the SAPs released by Scenedesmus sp. LX1 during the growth process. Resin fractionation was used firstly to divide the SAPs into six resin fractions, and then size exclusion chromatography (SEC) was performed to separate each resin fraction into several subfractions with different molecular weights (MW). The fingerprint graph integrated the information revealed by resin fractionation as well as that by SEC, showing that main components of SAPs changed from the hydrophilic subfractions with MW of 102-103 Da to the hydrophobic subfractions with MW of 103-104 Da. Comparing these results with the EEM fluorescence spectra of different resin fractions, the change of the main matters within SAPs could be inferred. |
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| Keywords: | Microalgae Soluble algal products Fingerprint analysis Resin fractionation Molecular weight distribution |
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