RNA is more UV resistant than DNA: the formation of UV-induced DNA lesions is strongly sequence and conformation dependent |
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| Authors: | Kundu Lal Mohan Linne Uwe Marahiel Mohamed Carell Thomas |
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| Affiliation: | Department Chemie, Ludwig-Maximilians University, Butenandtstrasse 5-13, 81377 Munich, Germany. |
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| Abstract: | DNA and RNA hairpins, which represent well-folded oligonucleotide structures, were irradiated and the amount of damaged hairpins was directly quantified by using ion-exchange HPLC. The types of photoproducts formed in the hairpins were determined by ESI-HPLC-MS/MS experiments. Irradiation of hairpins with systematically varied sequences and conformations (A versus B) revealed remarkable differences regarding the amount of photolesions formed. UV-damage formation is, therefore, a strongly sequence and conformation dependent process. |
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| Keywords: | DNA damage DNA repair DNA hairpin structures RNA UV irradiation |
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