Determination of Binding Constants for Basic Drugs with Serum Albumin by Affinity Capillary Electrophoresis with the Partial Filling Technique |
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Authors: | Hui Xu Xiao-Dong Yu Xu-Dong Li Hong-Yuan Chen |
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Institution: | (1) Key Laboratory of Analytical Chemistry for Life Science, Education Ministry of China, Department of Chemistry, Nanjing University, Nanjing, 210093, P.R. China |
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Abstract: | A simple, rapid, and accurate method is presented for determination of the binding constants of eight basic drugs with serum albumin by affinity capillary electrophoresis with the partial filling technique. Molecular modeling and multivariate regression analysis were used to investigate the relationship between the binding constants and the physicochemical properties of the drugs. The results show that hydrophobic attraction is responsible for interaction of most of the drugs with HAS, although interaction between sulfamethoxazole and HSA is because of electrostatic attraction. |
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Keywords: | Capillary electrophoresis Affinity electrophoresis Determination of binding constant Partial filling technique Serum albumin |
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