Preparation and biodistribution of [67Ga]-DTPA-rituximab in normal rats |
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Authors: | A R Jalilian L Mirsadeghi R Haji-Hosseini |
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Institution: | (1) Cyclotron and Nuclear Medicine Department, Nuclear Research Center for Agriculture and Medicine (NRCAM), Atomic Energy Organization of Iran (AEOI), P.O. Box, 31485-498 Karaj, Iran;(2) Biochemistry Department, Payam-e-Noor University, District 10, Tehran, Iran |
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Abstract: | Rituximab was successively labeled with 67Ga]-gallium chloride after residulation with freshly prepared cyclic DTPA-dianhydride. The best results of the conjugation
were obtained by the addition of 1 ml of a rituximab pharmaceutical solution (5 mg/ml, in phosphate buffer, pH 8) to a glass
tube pre-coated with DTPA-dianhydride (0.01 mg) at 25 °C with continuous mild stirring for 30 minutes. Radio thin-layer chromatography
showed an overall radiochemical purity of 96–99% at optimized conditions (specific activity = 300–500 MBq/mg, labeling efficiency
77%). The final isotonic 67Ga-DTPA-rituximab complex was checked by gel electrophoresis for radiolysis control. Radio-TLC was performed to ensure the
formation of only one species, followed by filtration through a 0.22 μm filter. Preliminary in vivo studies in normal rat
model was performed to determine the biodistribution of the radioimmunoconjugate up to 6 hours. |
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