气相色谱-串联质谱法快速测定调味品中3种糠醛类物质

建立了同时测定调味品中糠醛、5-甲基糠醛和5-羟甲基糠醛的气相色谱-串联质谱法(GC-MS/MS)。对样品的前处理方法及GC-MS/MS分析条件进行优化,样品经乙酸乙酯萃取,GC-MS/MS多反应监测模式(MRM)进行测定。3种糠醛类物质在0.001～20 mg/L范围内均呈良好的线性关系,相关系数为0.999 0～0.999 5;检出限(S/N=3)均为0.005 mg/kg,加标回收率为86%～95%。该法简便、快速、溶剂用量少,可消除调味品中复杂基质的干扰,结果准确可靠、灵敏度高,适用于调味品中3种糠醛类物质的同时测定。

Determination of Three Furfurals in Condimen by Gas Chromatography-Tandem Mass Spectrometry

A method for the determination of furfural,5-methylfurfural and 5-hydroxymethylfurfural in condimen by gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed.In this paper,the conditions for sample pretreatment and GC-MS/MS were investigated and optimized.The samples were extracted with ethyl acetate.The detection was carried out by GC-MS/MS under the multiple-reaction monitoring(MRM) mode.Good linear relationships were obtained in the concentration range of 0.001-20 mg/L of three analytes,with correlation coefficients of 0.999 0-0.999 5.The limits of detections(S/N=3) of three compounds were all 0.005 mg/kg,and the recoveries ranged from 86% to 95%.This method showed the advantages of simplicity,rapidness and low solvent consumption,and the complex matrix disturbance in condimen samples could be eliminated when MRM mode was adopted.