|
|||||
|
|
Enzymatic hydrolysis of pectic substances by gel-permeation chromatography |
|
| Authors: | Carunchio V. Girelli A. M. Sinibaldi M. Tarola A. M. |
| Affiliation: | (1) Dipartimento di Chimica, Universita degli Studi !["ldquo"]("/content/q1500g2603314871/xlarge8220.gif") La Sapienza!["rdquo"]("/content/q1500g2603314871/xlarge8221.gif") , P. le Aldo Moro 5, 00185 Rome, Italy;(2) Istituto di Cromatografia del CNR, Area della Ricerca di Roma, Monterotondo Stazione, C.P. 10, 00016 Roma, Italy |
| Abstract: | Summary A rapid liquid chromatographic (HPLC) method for the determination of pectinolytic activity of enzymes produced by Aspergillus Niger and Rhizopus species is reported.Compared with more conventional methods, HPLC is more reliable and has a much improved maximum sensitivity. The limit of quantitation of galacturonic acid is 0.1 g. |
| Keywords: | Column liquid chromatography Gel-permeation Pectinase activity Aspergillus Niger andRhizopus |
| 本文献已被 SpringerLink 等数据库收录！ | |