Adsorption of firefly luciferase at interfaces studied by total internal reflection fluorescence spectroscopy |
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Authors: | Vladimir Hlady Ping-Yang Yeh J. D. Andrade |
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Affiliation: | (1) Department of Bioengineering MEB 2480, University of Utah, 84112 Salt Lake City, Utah;(2) Department of Material Science and Engineering, University of Utah, 84112 Salt Lake City, Utah;(3) Present address: Ruder Boskovic Institute, Zagreb, Yugoslavia |
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Abstract: | The adsorption of luciferase onto silica surfaces was studied by total internal reflection fluorescence (TIRF) spectroscopy. Two model surfaces were used: hydrophilic and hydrophobic silica. Luciferase adsorbed differently on these two surfaces. Initial kinetics of luciferase adsorption onto the hydrophilic surface showed that luciferase adsorbs over an adsorption energy barrier of 3 kT The quantum yield of luciferase fluorescence decreased at the hydrophilic silica surface, which indicated that the protein conformation was altered during adsorption. Luciferase adsorption onto the hydrophobic silica surface proceeded with a small adsorption energy barrier and the fluorescence efficiency of adsorbed protein remained unchanged after adsorption. The affinity of luciferase for luciferin was measured using quenching of luciferase fluorescence with luciferin. The binding constant of the adsorbed luciferase-luciferin complex at the hydrophilic silica surface was two orders of magnitude smaller than the respective binding constant in the solution. Adsorbed luciferase showed an absence of ATP-dependent visible luminescence, indicating that the adsorbed enzyme was not active at either of the two silica surfaces. |
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Keywords: | Adsorption luciferase luciferin fluorescence quenching |
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