Quantification of tryptic peptides in quadrupole ion trap using high-mass signals derived from isotope-coded N-acetyl dipeptide tags |
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Authors: | Seo Jongcheol Yoon Hye-Joo Shin Seung Koo |
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Institution: | (1) Department of Chemistry, Bio-Nanotechnology Center, Pohang University of Science and Technology, Pohang, Kyungbuk, 790–784, Korea; |
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Abstract: | Isotope-labeled N-acetyl dipeptides (Ac-Xxx-Ala) are coupled to the primary amines of tryptic peptides and then analyzed by tandem mass spectrometry.
Amide bond cleavage between Xxx and Ala provides both low- and high-mass isotope-coded signals for quantification of peptides.
Especially, facile cleavage at the modified lysine side chain yields very strong high-mass quantitation signals in a noise-free
region. Tagging tryptic peptides with isobaric N-acetyl dipeptides is a viable strategy for accurate quantification of proteins, which can be used with most quadrupole ion
trap mass spectrometers carrying the 1/3 mass cut-off problem. |
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