| 高效液相色谱-脉冲安培检测法测定脑微透析溶液中单胺类神经递质 |
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| 引用本文： | 徐桂芸,常溪燕,常理文. 高效液相色谱-脉冲安培检测法测定脑微透析溶液中单胺类神经递质[J]. 分析化学, 2001, 29(2): 199-201 |
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| 作者姓名： | 徐桂芸 常溪燕 常理文 |
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| 作者单位： | 中国科学院化学研究所,分子科学中心, |
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| 基金项目： | 国家自然科学基金资助项目!（No．29775027） |
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| 摘 要： | 详细对比了脉冲安培与恒电位安培检测单胺类神经递质的响应值，结果表明冲安培检测上述物质的响应值是恒电位安培检测的1.2倍。建立了以3.4 -二羟基苄胺（DHBA）为内标的定量方法。在微透析溶液中的微量神经递质的回收率在74％－101％之间。
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| 关 键 词： | 单胺类神经递质 脉冲安培检测 高效液相色谱 脑微透析液 测定 |
Determination of Monoamine Neurotransmitters and Related Compounds in Microdialysis Solution by High Performace Liquid Chromatography with Pulsed Amperometric Detection |
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| Xu Guiyun,Chang Xiyan,Chang Liwen. Determination of Monoamine Neurotransmitters and Related Compounds in Microdialysis Solution by High Performace Liquid Chromatography with Pulsed Amperometric Detection[J]. Chinese Journal of Analytical Chemistry, 2001, 29(2): 199-201 |
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| Authors: | Xu Guiyun Chang Xiyan Chang Liwen |
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| Abstract: | A method for determination of monoamine neurotransmitters and related compounds in microdialysis solution by high performance liquid chromatography (HPLC) with pulsed amperometric detection was described.A compasrison of response of catecholamines betwenn iso potential amperometric (AD) and pulsed amperometric detection (PAD) was made. The results indicated that the sensitivity of PAD was 1.2 fold that of AD. The quantitative determination of monoamine neurotransmitters and related compounds was carried out with 3,4- dihydroxybenzylamine (DHBA) as internal standerd.The recoveries of monoamine neurotransmitters in microdialysis solution are 71% ~ 101%. |
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| Keywords: | Neurotransmitters pulsed amperometric detection higher performace liquid chromatography |
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