Electrochemical and Biological Studies on Reactivity of [VO(oda)(H2O)2], [Co(oda)(H2O)2]·H2O,and [Ni(oda)(H2O)3]·1.5H2O Towards Superoxide Free Radicals |
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| Authors: | Dariusz Wyrzykowski Iwona Inkielewicz‐St?pniak Justyna Czupryniak Dagmara Jacewicz Tadeusz Ossowski Michal Wo?niak Lech Chmurzyński |
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| Institution: | 1. Faculty of Chemistry, University of Gdańsk, Sobieskiego 18/19, 80–952 Gdańsk, Poland;2. Department of Medical Chemistry, Medical University of Gdańsk, D?binki 1, 80–211, Gdańsk, Poland |
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| Abstract: | The reactivity of superoxide free radicals (O2 · –) generated electrochemically towards the oxydiacetate metal complexes, namely VO(oda)(H2O)2], Co(oda)(H2O)2] · H2O, and Ni(oda)(H2O)3] · 1.5H2O (oda = oxydiacetate) was examined by cyclic voltammetry. The measurements were carried out in DMSO solution using a platinum electrode. Based on the height of the anodic peak Ea that corresponds to electrochemical oxidation O2 · – → O2 + e, in the absence and in the presence of the compounds in the mixture, their O2 · – scavenge ability was assessed. The influence of the type of the complex was briefly discussed. H2O2 was used to induce cellular injury in a mouse hippocampal cell line (HT22). The cytoprotection of chemical compounds was tested at the mitochondrial (MTT test) and plasma membrane level (LDH leakage). Dose‐dependent effect (10 and 100 μM of the complex) of investigated compounds was observed. |
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| Keywords: | Oxydiacetate metal complexes Free radicals Cyclic voltammetry Cytoprotection |
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