INTERACTION BETWEEN DNA AND 8–METHOXYPSORALEN STUDIED BY LINEAR DICHROISM |
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| Authors: | Folke Tjerneld Bengt Nordén Bo Ljunccren† |
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| Institution: | Departments of Biochemistry 1 and Inorganic Chemistry 1;, University of Lund. Chemical Center, S-220 07 Lund. Sweden ?Department of Dermatology. University of Lund, General Hospital, S-214 01 Malmö. Sweden |
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| Abstract: | Abstract— High sensitivity linear dichroism on DNA oriented by flow is shown to provide conclusive information about the interaction with 8–methoxypsoralen (8–MOP) both in the dark and by irradiation. 8–MOP forms directly in the dark a weak complex with DNA with a structure consistent with the intercalation model of Lerman. When aqueous DNA-psoralen solutions are UV irradiated (30800 nm). a photoadduct is formed. By comparison with experiments in ice matrix, it is suggested that it is the primarily bound 8–MOP that is responsible for the final product. It is inferred that this product is the 4 1 ,5 I -photoadduct of 8–MOP. The orientation of the photo adduct on DNA is not consistent with intercalation. |
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