高效液相色谱-荧光分析法同时测定人体尿液中黄蝶呤与异黄蝶呤

建立了高效液相色谱-荧光法同时测定癌症病人尿液中黄蝶呤及异黄蝶呤的新方法。选择荧光检测波长λex=345nm,λem=420nm。以磷酸盐缓冲溶液(pH=7.5)-甲醇(体积比为98∶2)为流动相,流速1.0mL/min,黄蝶呤与异黄蝶呤含量分别在0.0013～0.945μg/mL及0.00017～0.118μg/mL范围内与色谱峰面积呈良好的线性关系,线性相关系数分别为0.9999和0.9996,检出限分别为0.5ng/mL和0.05ng/mL,加标平均回收率在86.2%～107.5%之间。方法应用于癌症病人尿样分析,取得了较好的结果。

Determination of Xanthopterin and Isoxanthopterin in Human Urine by High Performance Liquid Chromatography with Fluorescence Detection

The high performance liquid chromatography (HPLC) with fluorescence detection method was developed for the determination of xanthopterin and isoxanthopterin in human urine. The mobile phase was phosphate excitation and emission wavelengths were 345 nm and 420 nm, respectively. The linear ranges of xanthopterin and isoxanthopterin were 0.0013 μg/mL～0.945 μg/mL and 0.00017 μg/mL～0.118 μg/mL with the linear regression equations of y=3×10~6x+28959 (r=0.9999) and y=1×10~8x-162133 (r=0.9996), respectively. The detection limits for xanthopterin and isoxanthopterin were 0.5 ng/mL and 0.05 ng/mL and the recoveries were in the range of 86.2%～107.5%. The proposed method was successfully applied to the determination of xanthopterin and isoxanthopterin in human urine.