组装式微流控系统制备双重包裹微液滴的方法研究

介绍了一种组装式微流控系统制备单、双重包裹微液滴的方法。微系统中用三通接头构成T型微流体通道,使得分散相在连续相强烈的剪切力和压力差作用下断裂形成单个微米级液滴。在制备单个微液滴基础上,用毛细管将两个三通接头串联,通过调控三相流量,可产生双重包裹液滴。结合实验结果,分析了流体粘度比对液滴大小的影响,并得出液滴的尺寸与流量比之间的关系式,为制备不同尺寸的液滴提供了参考依据。对制备的样品进行统计分析,结果显示,液滴的多分散性指数均小于3.2%,表明微液滴的高度均匀性。此外,通过调节三相液体的流量不仅可以控制内外层液滴的大小,还可以调节内层包裹液滴的个数。本文提出的制备方法,设备组装拆卸简便,不需表面亲疏水性处理,装置利用率高,产生的单、双重包裹微液滴可满足高通量的测量分析要求。

On A Preparation Method for Double Package Micro Droplet Based on Assembled Microfluidics

This paper introduces a method for preparation of single or double encapsulated droplet by using an assembled microfluidic system.In the micro system,a three-way joint is used to form Tshape microfluidic channel,which makes the dispersed phase fissure to form a uniform micro droplets due to the strong shear stress and pressure difference between dispersed phase and continuous phase.On the basis of single droplet preparation,connecting two three-way joints with glass capillary tube,double encapsulated droplet was directly formed through adjusting three-phase flow rates.According to experimental results,the influence of microfluidic viscosity ratio on the droplet size was preliminarily analyzed.The relational expression between droplet size and flowrate was obtained,which provides a reference for preparing droplets with various sizes.Statistical analysis on our results show that the polydispersity index is less than 3.2％,which indicates that prepared droplets have high uniformity.Besides,the inner and outer droplet size and encapsulated droplet number can be controlled independently just by adjusting flow rates.The advantage of this method is that its device can be easily assembled and disassembled,without particular hydrophillic surface treatment and high repetition of utilization.This method can be used for large-scale production of highly uniform single and double encapsulation droplets.