Analysis of 17β-Estadiol from Sewage in Coastal Marine Environment by Surface Plasmon Resonance Technique |
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Authors: | ZHANG Wei-wei CHEN Yu-chun LUO Zhao-feng WANG Ju-ying MA De-yi |
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Abstract: | In this study, surface plasmon resonance(SPR) for monitoring 17β-eatradiol( E2 ) was developed. The small molecule E2 was immobilized on a CM5 sensor chip for an indirect competitive immunoassay to detect F2. The SPR response based on the antigen-antibody reaction was measured by injecting the sample solution into the flow system.The limitation of detection was 0.445 μg/L. The developed SPE-SPR system was applied to analyze the seawater samples. Recovery of E2 was 91.6%-93.1%. Relative standard deviations(RSD) for the E2 assay were between 10.9%-15.1% ( n = 3). The range of determination of E2 samples from the sewage in the coastal marine environment was between ND(lower than detection limit) and ca. 11.78 ng/L. |
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Keywords: | Surface plasmon resonance(SPR) Immunoassay SPE |
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