生物碱的高效液相色谱分离分析与纯化制备研究进展

生物碱是天然产物中药用活性较好的一类化合物,在分离科学与技术领域,生物碱的分离一直是一个研究热点和难点问题。近年来,随着高效液相色谱填料和分离方法的发展,生物碱的分离分析和纯化制备有了长足的进步。该文主要针对碱性化合物的峰形拖尾问题,综述了高效液相色谱理论的发展和色谱分离技术的进步,以及近年来新型色谱填料和分离方法在生物碱分离分析和纯化制备中的应用,并对其前景进行了展望。

Research Progress on High Performance Liquid Chromatography in Analysis and Preparation of Alkaloids

Alkaloids are a class of natural compounds with good pharmacological properties.In the field of separation science and technology,alkaloid separation has always been a hot and difficult problem.In recent years,with the development of high performance liquid chromatography(HPLC) materials and separation methods,great progresses in alkaloid analysis and preparation have been achieved.In this article,the theoretical development and technological advances with respect to peak tailing problems of basic compounds are summarized,and the applications of HPLC in natural alkaloid analysis and preparation are discussed.The further development of HPLC separation on alkaloids is also looked forward.