流动注射-化学发光法测定猪饲料中的吡喹酮

基于吡喹酮对Luminol-NaIO4化学发光体系有较强的抑制作用,并结合流动注射分析技术,建立了测定吡喹酮的流动注射-化学发光(FI-CL)新方法。在最佳实验条件下,吡喹酮在4.0×10^-8~1.0×10^-6mol/L浓度范围内与相对化学发光强度呈良好的线性关系,该方法对测定吡喹酮显示出较高的灵敏度,检出限(LOD,3σ)为9.9×10^-9mol/L。对2.0×10^-7mol/L的吡喹酮平行测定11次,其相对标准偏差(RSD)为1.4%。采用该方法对猪饲料样品进行加标实验,回收率为98.5%~100%。该方法已经成功用于猪饲料样品中吡喹酮的测定。

Determination of Praziquantel in Pig Feed by Flow Injection Chemiluminescence

A novel flow injection-chemiluminescence(FI-CL) method was established for the determination of praziquantel based on the strong inhibition of praziquantel on Luminol-NaIO 4 chemiluminescence system.Under the optimal experimental conditions,there was a good linear relationship between relative intensity of chemiluminescence and praziquantel in the concentration range of 4.0×10^-8 -1.0×10^-6 mol/L,with a limit of detection(LOD) of 9.9×10^-9 mol/L.The relative standard deviation(RSD, n =11) was 1.4 % for the determination of 2.0×10^-7 mol/L praziquantel.The proposed method was used for the detection of praziquantel in the spiked pig feed sample with recoveries of 98.5 %-100 %.The method was successfully applied in the determination of praziquantel in pig feed with satisfactory results.