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Chemiluminescent optical fiber immunosensor for the detection of anti-West Nile virus IgG |
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| Authors: | Herrmann Sebastien Leshem Boaz Landes Shimi Rager-Zisman Bracha Marks Robert S |
| Affiliation: | a Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel b The Institute of Applied Biosciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel c Department of Microbiology and Immunology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel d Center for Cancer Research, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel |
| Abstract: | An ELISA-based optical fiber methodology developed for the detection of anti-West Nile virus IgG antibodies in serum was compared to standard colorimetric and chemiluminescent ELISA based on microtiter plates. Colorimetric ELISA was the least sensitive, especially at high titer dilutions. The fiber-optic immunosensor based on the same ELISA immunological rationale was the most sensitive technique. |
| Keywords: | Optical fiber immunosensor ELISA West Nile virus Chemiluminescence |
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