Selective spectrofluorimetric method for paracetamol determination through coumarinic compound formation |
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Authors: | Oliva María de los A Olsina Roberto A Masi Adriana N |
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Institution: | a Área de Química Analítica “Dr. Carlos B. Marone”, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina b Área de Bromatología, Ensayo y Valoración de Medicamentos, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina c Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Chacabuco y Pedernara, 5700 San Luis, Argentina |
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Abstract: | A spectrofluorimetrical selective method was designed for determination of paracetamol in tablets. This important technique can be characterized by its sensitivity, simplicity, celerity and cheaper cost than current official methods. The employed methodology involves coumarinic compound formation obtained by reaction between paracetamol and ethylacetoacetate (EAA) in the presence of sulphuric acid as catalyst. The reaction product is highly fluorescent at 478 nm, being excited at 446 nm.The linear concentration range of the application was 0.1-0.4 μg/ml of paracetamol and the detection limit was 57 ng/ml.The influence of different variables was studied and optimized through chemometric techniques. Applying the above-mentioned method good results were obtained with regard to pharmaceutical formulations containing paracetamol. Therefore, it is relevant to suggest this profitable technique for medicament control analysis. |
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Keywords: | Fluorescence Paracetamol Ethylacetoacetate Coumarin |
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