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Towards single-spot multianalyte molecular beacon biosensors |
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| Authors: | Strohsahl Christopher M Du Hui Miller Benjamin L Krauss Todd D |
| Institution: | a Department of Biochemistry and Biophysics, The Center for Future Health, University of Rochester, Rochester, NY, USA b Department of Chemistry, The Center for Future Health, University of Rochester, Rochester, NY, USA c Department of Dermatology, The Center for Future Health, University of Rochester, 601 Elmwood Avenue, Box 697 Rochester, NY 14642, USA |
| Abstract: | The separate developments of microarray patterning of DNA oligonucleotides, and of DNA hairpins as sensitive probes for oligonucleotide identification in solution, have had a tremendous impact on basic biological research and clinical applications. Herein, we will discuss several successful efforts to develop oligonucleotide sensors based on the surface immobilization of functionalized DNA hairpins. We also will discuss the development of prototypical single-spot multianalyte “Molecular Beacon” biosensors. Importantly, we show that organic fluorophores will likely be inadequate in moving this technology forward and new approaches, such as the use of nanotechnology, will be needed. |
| Keywords: | Biosensor Molecular beacon Nanocrystals Single-base mismatch Microarray |
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