Determination of arsenic compounds in beverages by high-performance liquid chromatography-inductively coupled plasma mass spectrometry |
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Authors: | Coelho N M M Coelho L M Lima E S de Pastor A Guardia M de la |
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Affiliation: | a Institute of Chemistry, University of Uberlândia, Av. João Naves de Ávila, 2160, Campus Santa Mônica, CEP: 38.408.110 Uberlândia, MG, Brazil b Institute of Chemistry, University of Campinas, P.O. Box 6154, CEP: 13.083.970, Campinas, SP, Brazil c Department of Geology, University of Pernambuco, Av. Acad. Hélio Ramos S/N, Cidade Universitária, CEP: 50.740-530, Recife, PE, Brazil d Department of Analytical Chemistry, University of Valencia, 50 Dr. Moliner St. 46100 Burjassot, Valencia, Spain |
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Abstract: | Arsenic compounds including arsenous acid (As(III)), arsenic acid (As(V)), dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) were separated by high-performance liquid chromatography (HPLC) and detected by inductively coupled plasma mass spectrometry (ICP-MS). A Hamilton PRX-100 anionic-exchange column and a pH 8.5 K2HPO4/KH2PO4 5.0 × 10−3 mol L−1 mobile phase were used to achieve arsenic speciation. The separation of arsenic species provided peaks of As(III) at 2.75 min, DMA at 3.33 min, MMA at 5.17 min and As(V) at 12.5 min. The detection limits, defined as three times the standard deviation of the lowest standard measurements, were found to be 0.2, 0.2, 0.3 and 0.5 ng mL−1 for As(III), DMA, MMA and As(V), respectively. The relative standard deviation values for a solution containing 5.0 μg L−1 of As(III), DMA, MMA and As(V) were 1.2, 2.1, 2.5 and 3.0%, respectively. This analytical procedure was applied to the speciation of arsenic compounds in drinking (soft drink, beer, juice) samples. The validation of the procedure was achieved through the analysis of arsenic compounds in water and sediment certified reference materials. |
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Keywords: | Arsenic speciation HPLC-ICP-MS Beverages |
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