Preparation and evaluation of a large-volume radial flow monolithic column |
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Authors: | Yang Changlong Wei Yuanlong Zhang Qinghe Zhang Weibing Li Tong Hu Haoquan Zhang Yukui |
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Institution: | a School of Chemical Engineering, Dalian University of Technology, Dalian 116024, China b Dalian Elite Analytical Instrument Co. Ltd., Dalian 116011, China c School of Chemical Engineering, Qiqihar University, Qiqihar 161006, China d Nanjing University of Science and Technology, Nanjing 210094, China |
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Abstract: | In this study, a 38 mL monolith with homogeneous porous structure was produced by a single polymerization from glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) in the presence of porogens and an initiator. The uniform temperature distribution within the reaction system was achieved by adding reactant mixture continuously and enhancing the heat transfer ability of the polymerization system. Homogeneous porous structure in the monolith was proved by SEM and the pore size distribution profiles measured by mercury intrusion porosimetry. Experimental results from proteins separation indicated that the dynamic capacity and resolution of radial flow monolithic column were independent of flow rates. Furthermore, the pressure drop on the column was linearly dependent on the flow rate and did not exceed 1.7 MPa even at a flow rate of 50 mL/min, which proved that the prepared monolith could be used in the quick separation and preparation of biopolymers. |
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Keywords: | Monolithic column GMA-EDMA Temperature controlling Porous structure Radial flow |
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