Microchip-based electrochromatography: designs and applications |
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| Authors: | Pumera Martin |
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| Affiliation: | Department of Chemistry, Universitat Autonoma de Barcelona, E-08193 Bellaterra, Barcelona, Spain |
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| Abstract: | Different techniques and methods of electrochromatography on “lab on a chip” devices are reviewed. Described approaches include open-channel microchip electrochromatography relying on C8, C18 and novel gold nanoparticle (GNP) coating of microchannel wall; packed-channel microchip electrochromatography with new ways of automated loading and unloading of conventional octadecylsilica beads; monolith-based microchip electrochromatography with tailored casting of stationary phase at the specific places of microfluidic network and novel photolitographically fabricated collocated monolithic structures. Specific issues related to the microchip electrochromatography, i.e. importance of high aspect ratio of the microchannels in the open-channel electrochromatography or approaches eliminating the wall effect in the monolith-based electrochromatography, are discussed. Various applications for environmental, pharmacological, genomic and proteomic analysis are described. The operation parameters of reviewed microsystems are summarized in easy-to-read tables. |
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| Keywords: | 2D, two-dimensional AA, acrylic acid AIBN, azo-isobutyronitrile AMPS, 2-acrylamido-2-methylpropanesulfonic acid β, aspect ratio BODIPY, 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-(S)-indacene COMOSS, collocate monolith support structures μCEC, microchip-based electrochromatography m-μCEC, monolithic microchip-based electrochromatography o-μCEC, open-channel microchip-based electrochromatography p-μCEC, packed-channel microchip-based electrochromatography μCZE, microchip-based zone electrophoresis EOF, electroosmotic flow GNP, gold nanoparticle H, theoretical plate height μLC, microchip-based liquid chromatography MES, 2-(N-morpholino)-ethanesulfonic acid MPTMS, 3-methacryloxypropyltrimethoxysilane MS, mass spectrometry ODS, octadecylsilica PAH, polycyclic aromatic hydrocarbon PDADMAC, poly(diallyldimethylammonium chloride) PDMS, poly(dimethylsiloxane) PSG, photopolymerized sol-gel PMMA, poly(methyl methacrylate) RP, reverse phase SDS, sodium dodecyl sulfate SSA, 4-styrenesulfonic acid StMA, stearyl methacrylate Tris, tris(hydroxymethyl)methylamine VSA, vinylsulfonic acid |
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