Dynamic coating for resolving rhodamine B adsorption to poly(dimethylsiloxane)/glass hybrid chip with laser-induced fluorescence detection |
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Authors: | Kang Jianzhen Yan Jilin Liu Jifeng Qiu Haibo Yin Xue-Bo Yang Xiurong Wang Erkang |
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Institution: | State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China |
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Abstract: | In this paper a method was described about dynamic coating for resolving rhodamine B (RB) adsorption on a hybrid poly(dimethylsiloxane) (PDMS)/glass chip. The results showed that when the non-ionic surfactant Triton X-100 was higher than 0.5% (v/v) into the phosphate buffer, the adsorption of RB appeared. Besides, some separation conditions for RB were investigated, including concentration of Triton X-100, concentration and pH value of running buffer, separation voltage and detection site. Through comparing electroosmotic flow, plate numbers and other parameters, an acceptable separation condition was obtained. Under optimized conditions, the precisions of RB detection (R.S.D., n = 10) were 2.62% for migration time, 4.78% for peak height respectively. Additionally, RB concentration linearity response was excellent with 0.9996 of correlation coefficient between 1 and 100 μM, and a limit of detection (S/N = 3) was 0.2 μM. Finally, we separated rhodamine B isothiocyanate and lysine deriving from the fluorescent probe, and the result displayed that the dynamic coating method was applicable by CE separations using PDMS/glass chip. |
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Keywords: | Dynamic coating Rhodamine B adsorption Poly(dimethylsiloxane) Triton X-100 |
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