Fluorescence detection of glutathione reductase activity based on deoxyribonucleic acid-templated silver nanoclusters |
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| Authors: | Shuyun Zhu Xian-en Zhao Wei Zhang Zhongyuan Liu Wenjing Qi Saima Anjum Guobao Xu |
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| Institution: | 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China;2. Shandong Provincial Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, Shandong, China;3. University of the Chinese Academy of Sciences, No. 19A Yuquanlu, Beijing 100049, China;4. Department of Chemistry, Faculty of Science, The Islamia University of Bahawalpur, 63100, Pakistan |
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| Abstract: | Fluorescent silver nanoclusters stabilized by DNA (DNA-AgNCs) exhibit distinct response rates to thiol and disulfide. Glutathione reductase can catalyze the reduction of the oxidized glutathione (GSSG) quickly to reduced glutathione (GSH) in the presence of β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH). Consequently, DNA-AgNCs can serve as a new fluorescent platform for assaying the glutathione reductase (GR) activity. This newly proposed assay has a high sensitivity and a good selectivity toward GR. The GR activity can be detected in the range of 0.2–2.0 mU mL−1 with a minimum detectable concentration of 0.2 mU mL−1. Pepsin, lysozyme, trypsin, avidin, thrombin, myoglobin, and BSA have little effect on the fluorescence intensity of DNA-AgNCs. The GR activity assay is successfully used to monitor the inhibition of GR activity by a typical inhibitor 1,3-bis(2-chloroethyl)-1-nitrosourea. |
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| Keywords: | Glutathione reductase Activity Silver nanoclusters Fluorescence Deoxyribonucleic acid |
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