Simultaneous determination of amino acids and carbohydrates in culture media of Clostridium thermocellum by valve-switching ion chromatography |
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| Authors: | Yun Fa Haiyan Yang Chengshuai Ji He Cui Xinshu Zhu Juan Du Jun Gao |
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| Institution: | 1. Public Laboratory of Bioenergy and Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China;2. China University of Petroleum, No.66, West Changjiang Road, Qingdao Economic & Technological Development Zone 266580, China;3. Technical Center of Shandong Entry-Exit Inspection and Quarantine Bureau, No. 70 Qutangxia Road, Qingdao 266002, China;4. Metabolomics group, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China;5. College of Materials Science and Engineering, Qingdao University of Science & Technology, No. 53 Zhengzhou Road, Qingdao 266042, China |
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| Abstract: | An improved method for the simultaneous determination of 20 amino acids and 7 carbohydrates using one-valve switching after injection, ion chromatography, and integrated pulsed amperometric detection is proposed. The resolution of the amino acids and carbohydrates in the cation trap column was investigated. In addition, parameters including flow liquid type, flow rate, concentration, and valve-switch timing were optimized. The method is time-saving, effective, and accurate for the simultaneous separation of amino acids and carbohydrates, with a mean correlation coefficient of >0.99 and repeatability of 0.5–4.6% for eight replicates. The method was successfully applied in the analysis of amino acids and carbohydrates in aseptic media and in extracellular culture media of three phenotypes of Clostridium thermocellum. |
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| Keywords: | Ion chromatography Valve switching Amino acids Carbohydrates Electrochemical detector |
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