Sulfate-reducing bacteria detection based on the photocatalytic property of microbial synthesized ZnS nanoparticles |
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Authors: | Peng Qi Dun Zhang Yi Wan |
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Institution: | 1. National Engineering Research Center for Marine Corrosion Protection, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China;2. University of the Chinese Academy of Sciences, 19 (Jia) Yuquan Road, Beijing 100039, China |
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Abstract: | This work presented a novel method for specific detection of sulfate-reducing bacteria (SRB) based on the photocatalytic property of ZnS nanoparticles. ZnS semiconductor nanoparticles were synthesized by taking advantage of the characteristic bacterial metabolite, sulfide, and then ZnS nanomaterials were used as photocatalyst for methylene blue (MB) photodegradation. As the amount of ZnS photocatalyst synthesized from microbe metabolized sulfide was affected by initial bacterial concentration before cultivation, the photodegradation ratio of MB was highly related with initial SRB concentration. Under the optimized conditions, a linear relationship between the MB photodegradation ratio and the logarithm of SRB concentration was observed in the range of 1.0 × 103–1.0 × 108 cfu mL−1. Besides, this proposed method showed excellent specificity for SRB detection. To the best of our knowledge, this is the first example of using the photocatalytic property of microbial synthesized ZnS for bacterial detection. |
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Keywords: | Sulfate-reducing bacteria Zinc sulfide Photocatalytic Photodegradation Nanoparticles |
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