A label-free electrochemical biosensor based on a DNA aptamer against codeine |
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| Authors: | Liangliang Huang Xiaojuan Yang Cui Qi Xiaofang Niu Chunling Zhao Xiaohui Zhao Dihua Shangguan Yunhui Yang |
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| Institution: | 1. Chemistry and Chemical Engineering College, Yunnan Normal University, Kunming 650092, China;2. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China |
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| Abstract: | In order to develop a sensor for opium alkaloid codeine detection, DNA aptamers against codeine were generated by SELEX (systematic evolution of ligands by exponential enrichment) technique. An aptamer HL7-14, which is a 37-mer sequence with Kd values of 0.91 μM, was optimized by the truncation-mutation assay. The specificity investigation shows that HL7-14 exhibits high specificity to codeine over morphine, and almost cannot bind to other small molecule. With this new selected aptamer, a novel electrochemical label-free codeine aptamer biosensor based on Au-mesoporous silica nanoparticles (Au-MSN) as immobilized substrate has been proposed using Fe(CN)6]3−/4− as electroactive redox probe. The linear range covered from 10 pM to 100 nM with correlation coefficient of 0.9979 and the detection limit was 3 pM. Our study demonstrates that the biosensor has good specificity, stability and well regeneration. It can be used to detect codeine. |
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| Keywords: | Codeine Aptamer Systematic evolution of ligands by exponential enrichment Electrochemical aptasensor Au-mesoporous silica nanoparticles |
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