Solid phase microextraction fills the gap in tissue sampling protocols |
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| Authors: | Barbara Bojko Krzysztof Gorynski German Augusto Gomez-Rios Jan Matthias Knaak Tiago Machuca Vinzent Nikolaus Spetzler Erasmus Cudjoe Michael Hsin Marcelo Cypel Markus Selzner Mingyao Liu Shaf Keshavjee Janusz Pawliszyn |
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| Institution: | 1. Department of Chemistry, University of Waterloo, Waterloo, ON, Canada;2. Department of Surgery, Multi Organ Transplant Program, Toronto General Hospital, Toronto, ON, Canada;3. Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, University Health Network and Department of Surgery, University of Toronto, Toronto, ON, Canada |
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| Abstract: | Metabolomics and biomarkers discovery are an integral part of bioanalysis. However, untargeted tissue analysis remains as the bottleneck of such studies due to the invasiveness of sample collection, as well as the laborious and time-consuming sample preparation protocols. In the current study, technology integrating in vivo sampling, sample preparation and global extraction of metabolites – solid phase microextraction was presented and evaluated during liver and lung transplantation in pig model. Sampling approaches, including selection of the probe, transportation, storage conditions and analyte coverage were discussed. The applicability of the method for metabolomics studies was demonstrated during lung transplantation experiments. |
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| Keywords: | In vivo SPME Metabolomics Tissue sampling Surgery |
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