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Molecularly imprinted polymers with assistant recognition polymer chains for bovine serum albumin |
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| Authors: | GUO MinJie GAO Ting FAN Zhi YAO JingXia XIA JianJun & MI HuaiFeng |
| Affiliation: | GUO MinJie1,GAO Ting1,FAN Zhi1,YAO JingXia1,XIA JianJun2 & MI HuaiFeng2 1Department of Chemistry,College of Sciences,Tianjin University of Science , Technology,Tianjin 300457,China,2Key Laboratory of Functional Polymer Materials,Ministry of Education,Institute of Polymer Chemistry,Nankai University,Tianjin 300071 |
| Abstract: | A new protein molecularly imprinted polymer (MIP) was prepared with grafting polyvinyl alcohol as assistant recognition polymer chains (ARPCs). The ARPCs and acrylamide monomers were interpenetrated and then polymerized on the surface of macroporous acrylate adsorbent spheres. The template BSA was removed by treatment with 2.00 mol L-1 potassium chloride (KCl) solution and the adsorbed proteins were detected with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). 0.150, 0.500, and 2.00 mo... |
| Keywords: | polyvinyl alcohol assistant recognition polymer chains molecular imprinting protein |
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