| 家蚕微粒子虫表面增强拉曼光谱研究 |
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| 引用本文： | 伍铁桥 唐聘芳. 家蚕微粒子虫表面增强拉曼光谱研究[J]. 光散射学报, 1993, 5(2): 88-92 |
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| 作者姓名： | 伍铁桥 唐聘芳 |
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| 作者单位： | 四川大学，四川大学，四川大学，四川大学，四川省农科院蚕桑研究所 四川 成都 610064，四川 成都 610064，四川 成都 610064，四川 成都 610064，四川 南充 637000 |
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| 摘 要： | 本文报导并分析了家蚕微粒子虫(Nosema bombycis Naegeli,缩写为NbN)孢子在PH4.0条件下的表面增强拉曼光谱(SERS),在常规的实验条件下未得到NbN的一般拉曼谱,同时也未获得其它PH条件下的表面增强拉曼谱。对获得的NbN的SERS分析表明:NbN孢子通过S原子,COO~-和NH_2基团被吸附到银胶表面上,Tyr、Trp残基侧链靠近银表面,以Tyr残基侧链的振动增强效应最显著。当NbN孢子吸附在银胶表面时,NbN孢子中的C—C—S—S—C—C链为扭曲—扭曲—反式结构。
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| 关 键 词： | 家蚕微粒子虫 孢子 表面增强拉曼光谱 |
Surface Enhanced Raman Spectroscopy of the Nosema bombycis Naegeli |
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| Wu Tieqiao,Liu Xinming,Huang Tianquan,Bao Peidi,Sichuan University Chengdu Tang Pinfang,The Institute of Sericultural and Mulberry Sciences Under Sichuan Academia of Agricultural sciences Sichuan Nanchong. Surface Enhanced Raman Spectroscopy of the Nosema bombycis Naegeli[J]. Chinese Journal of Light Scattering, 1993, 5(2): 88-92 |
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| Authors: | Wu Tieqiao Liu Xinming Huang Tianquan Bao Peidi Sichuan University Chengdu Tang Pinfang The Institute of Sericultural Mulberry Sciences Under Sichuan Academia of Agricultural sciences Sichuan Nanchong |
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| Affiliation: | Wu Tieqiao,Liu Xinming,Huang Tianquan,Bao Peidi,Sichuan University Chengdu 610064Tang Pinfang,The Institute of Sericultural and Mulberry Sciences Under Sichuan Academia of Agricultural sciences Sichuan Nanchong 637000 |
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| Abstract: | The surface-enhanced Raman spectra of Nosema bombycis Naegeli absorbed on colloidal silver has been obtained and analyzed when PH value was 4.0. The common spectra can't be obtained under normal condition, as well as the SERS can't be get under other PH values. Analyzed the SERS of NbN un- der PH 4.0 and proved that: the NbN was absorbed on the silver surface with S-atom, carboxylate and amino groups. The side-chain of Tyr and Trp residues closed the silver surface. The vibration enhancement efficiency of Tyr residue side-chain was highest. The NbN absorbed on silver surface has gauche-gauche-trans conformation of C-C-S-S-C-C linkage. |
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| Keywords: | Nosema bombycis Naegeli Spore Surface-enhanced Raman Spectroscopy |
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