CHANGES IN FLUORESCENCE SPECTRA OF CHLOROPLASTS INDUCED BY A NATURALLY-OCCURRING FACTOR |
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Authors: | B. NATHANSON M. BRODY |
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Affiliation: | Department of Biological Sciences, Hunter College, New York, N.Y. 10021, U.S.A. |
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Abstract: | Abstract— –Fluorescence emission changes (measured at – 196°C) of Ricinus chloroplasts incubated in isolation medium, and of chloroplasts from algae and higher plants incubated in Ricinus leaf extract, are described. Such incubation results in a transformation of the three-banded emission spectrum (F735, F698, F685) into a virtually one-banded spectrum, with maximum at 698 nm. That these changes are a consequence of the conversion (deaggregation) of the form of chlorophyll giving rise to F735 into a form contributing to fluorescence at 698 nm is suggested on the basis of room temperature absorption and low temperature fluorescence excitation studies, made concomitantly with the low temperature emission studies. |
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