儿茶素对溶血卵磷脂胆碱所致细胞损伤的保护作用

作者以体外培养的小牛主动脉内皮细胞为材料，研究了溶血卵磷脂胆碱（LPC）和氧自由基（OFR）对血管内皮细胞（VEC）的损伤，及儿茶素对VEC的保护。结果显示：当VEC与LPC（6ug/ml）或黄嘌呤／黄嘌呤氧化酶（X／XO）（10umol/L 200umol/L）共孵育24小时时，表现为细胞内乳酸脱氢酶（LDH）泄漏量增多，细胞内过氧化脂质丙二醛（MDA）含量升高，细胞生长减缓，存活率下降；当加入不同浓度的儿茶素后则可明显抑制LDH的泄量，降低MDA含量，细胞生长正常，存活率提高。表明LPC与X／XO对VEC有损伤作用，而儿茶素则能通过抗氧化途径抵抗LPC与X／XO至VEC的损伤。

Protective Effects of Catechin on Vascular Endothelial Cells Against Lysophosphatidylcholine Damages

Effects of catechin on cultured bovine endothelial cells damaged by lysophosphatidylcholine(LPC) or oxygen free radicals(OFR) were investigated. When vascular endothelial cells(VEC) was incubated with LPC(5ug/ml) or xanthine/xanthine oxidase(X/XO) (10umol/L+200umol/L)for 24 hours, the contents of lactate dehydrogenase(LDH) and molondialdehyde(MDA) were markedly increased, the VEC grew slowly. Treatment with different concentrations of catechin caused a reduction in LDH and MDA contents, the VEC grew normally. There results suggest that catechin afforded protection against VEC damages induced by LPC or X/XO and that the protective effect of catechin may be due to anti-lipid peroxidation via free radicaed scavenging activity.