Study on the (R)-oxynitrilase activity of Pouteria sapota |
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| Authors: | Aida Solís Héctor Luna Norberto Manjarrez Herminia I Pérez |
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| Institution: | Departamento Sistemas Biológicos, Universidad Autónoma Metropolitana, Unidad Xochimilco. Calz. del Hueso No. 1100, Col Villa Quietud, Coyoacán, C.P. 04960 Mexico, D.F., Mexico |
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| Abstract: | Mamey (Pouteria sapota) defatted meal was used to catalyze the enantioselective addition of HCN to α,β-unsaturated aldehydes. Using a biphasic system of diisopropyl ether and citrate buffer (0.1 M, pH 5.0, 10% v/v), the (R)-cyanohydrins obtained showed good conversion (from 54 to 98%) and enantiomeric excess (from 74 to 99%). |
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| Keywords: | Oxynitrilases Cyanohydrins Mamey |
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