An RP-HPLC determination of 5-hydroxymethylfurfural in honey The case of strawberry tree honey |
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Authors: | Spano Nadia Casula Lucia Panzanelli Angelo Pilo Maria I Piu Paola C Scanu Roberta Tapparo Andrea Sanna Gavino |
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Institution: | a Università degli Studi di Sassari, Dipartimento di Chimica, via Vienna 2, 07100 Sassari, Italy b Università degli Studi di Padova, Dipartimento di Scienze Chimiche, via Marzolo 1, 35131 Padova, Italy |
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Abstract: | The use of the RP-HPLC official method of the International Honey Commission (IHC) for the determination of 5-hydroxymethylfurfural (HMF) in strawberry tree honey (Arbutus unedo, a typical Sardinian honey) has brought to light a specific and heavy chromatographic interference that prevents accurate quantification. The interference has been identified as homogentisic acid (HA), i.e. the marker of the botanical origin of the honey. For this reason, an alternative RP-HPLC method is proposed. The bias-free method allows a complete separation of HMF from HA to the baseline level and is faster and more precise than the RP-HPLC official method: the detection and quantification limits are 1.9 and 4.0 mg kg−1, respectively, whereas the repeatability is ca. 2% in the HMF concentration range of 5-140 mg kg−1. |
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Keywords: | Strawberry tree honey 5-Hydroxymethylfurfural Homogentisic acid RP-HPLC |
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