FTIR-determination of sterols from the red alga Asparagopsis armata: Comparative studies with HPLC |
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| Authors: | El Hattab Naïma Daghbouche Yasmina Hattab Mohamed El Piovetti Louis Garrigues Salvador Guardia Miguel de la |
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| Institution: | Laboratory of Molecular and Macromolecular Physical Chemistry, Department of Chemistry, University of Blida, P.O. Box 270 Soumâa, Blida 09000, Algeria |
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| Abstract: | An analytical procedure was developed for the determination of the total amount of sterols in the red alga Asparagopsis armata, globally determined as cholesterol, which is the major sterol contained in red algae. Samples, previously saponified with KOH were preconcentrated on DSC-18 solid phase cartridges (SPE) and eluted with dichloromethane stabilized with β-amylene. Fourier transform infrared (FTIR) spectrometry was employed for selective detection at 1049 cm−1 with a baseline established between 1000 and 1079 cm−1. The results were compared to those obtained by high performance liquid chromatography (HPLC). The concentration obtained in actual samples from alga was 3.37% (w/w) by FTIR and 3.30% (w/w) by HPLC, showing a good comparability between the two methods. |
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| Keywords: | Fourier transform infrared Solid phase extraction High performance liquid chromatography Sterol determination Red alga Asparagopsis armata |
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